| 质粒名称: | pGWB454 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 74836 |
| 存储实验室: | Tsuyoshi Nakagawa |
|
载体骨架基本信息 |
|
| 载体名称: | pPZP221 |
| 载体出品公司: | Hajdukiewicz P., Svab Z. and Maliga P. |
| 载体抗性: | 氯霉素和大观霉素 |
| 空载体大小: | 8500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 去除庆大霉素耐药标记物和卡那霉素耐药标记物（NOS发起人：NPTII：NOS终止符）已插入。 |
| 载体类型: | 植物表达 |
| 筛选标记: | 卡那霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CaMV35S |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGWB454 was a gift from Tsuyoshi Nakagawa (Addgene plasmid # 74836 ; http://n2t.net/addgene:74836 ; RRID:Addgene_74836) |
| 参考文献部分: | Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, Ishiguro S. Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7. 10.1271/bbb.70216 PubMed 17690442 |
| 相关质粒: |
pGWB4 pGWB5 pGWB11 pGWB12 pGWB17 pGWB18 pGWB21 pGWB406 pGWB453 pGWB454 pGWB455 pGWB504 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGWB454 12363 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Gateway cloning compatible binary vector for C-terminal fusion with
mRFP (CaMV35S promoter)..
ACCESSION .
VERSION .
KEYWORDS pGWB454
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12363)
AUTHORS Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata
R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T,
Ishiguro S
TITLE Improved Gateway binary vectors: high-performance vectors for
creation of fusion constructs in transgenic analysis of plants.
JOURNAL Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
PUBMED 17690442
REFERENCE 2 (bases 1 to 12363)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12363
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 54..78
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 266..288
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 280..297
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 281..297
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 812..1157
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 1100..1120
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
protein_bind 1179..1303
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 1340..1370
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 1424..2083
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(1490..1509)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 2425..2730
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 2609..2628
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(2771..2895)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 2906..3583
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind complement(3036..3054)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 3304..3323
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 3498..3521
/label=DsRed1-C
/note="DsRed1, forward primer"
terminator 3605..3857
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(3891..3907)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3891..3907)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3904..3926)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3915..3931
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3939..3969)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3984..4005
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
terminator 4039..4291
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(4681..5475)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4792..4811)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 5402..5421
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(5496..5679)
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
promoter complement(5532..5711)
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature 5885..5909
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 6430..7221
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 7467..8055
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7956..7975
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8209..8226
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 8241..8381
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(8307..8326)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8467..8489
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 8725..8919
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(8985..10058)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(10487..11116)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
1381 gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
1981 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
2401 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
2881 cttgtacaaa gtggttgata acagcatggc ctcctccgag gacgtcatca aggagttcat
2941 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
3001 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
3061 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc cagtacggct ccaaggccta
3121 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
3181 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
3241 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
3301 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc ggatgtaccc
3361 cgaggacggc gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca
3421 ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc
3481 ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
3541 acagtacgag cgcgccgagg gccgccactc caccggcgcc taagcttaga gctcgaattt
3601 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
3661 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
3721 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
3781 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
3841 atctatgtta ctagatcggg aattggttcc ggaaccaatt cgtaatcatg gtcatagctg
3901 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
3961 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc
4021 gcctatttct gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
4081 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
4141 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
4201 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
4261 agaaacttta ttgccaaatg tttgaacgat cggggatcat ccgggtctgt ggcgggaact
4321 ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt
4381 cgccgccgac gccgttgatg tggacgccgg gcccgatcat attgtcgctc aggatcgtgg
4441 cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc accttcgacc gcctgttccg
4501 cagagatccc gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
4561 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
4621 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
4681 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
4741 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
4801 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
4861 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
4921 gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
4981 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
5041 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
5101 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
5161 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
5221 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
5281 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
5341 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
5401 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
5461 atcttgttca atcatgcgaa acgatccaga tccggtgcag attatttgga ttgagagtga
5521 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
5581 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
5641 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa
5701 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
5761 ataacgtgac tcccttaatt ctccgctcat gatgaattca gaaataaatt cagcctaatt
5821 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
5881 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
5941 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
6001 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
6061 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
6121 atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt
6181 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
6241 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
6301 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
6361 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
6421 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
6481 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
6541 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
6601 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
6661 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
6721 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
6781 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
6841 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
6901 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
6961 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
7021 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
7081 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
7141 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
7201 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
7261 ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat
7321 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
7381 gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
7441 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
7501 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
7561 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
7621 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
7681 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
7741 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
7801 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
7861 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
7921 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
7981 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
8041 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
8101 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
8161 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
8221 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
8281 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
8341 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
8401 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
8461 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
8521 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
8581 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
8641 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
8701 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
8761 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
8821 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
8881 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
8941 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
9001 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
9061 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
9121 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
9181 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
9241 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
9301 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
9361 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
9421 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
9481 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
9541 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
9601 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
9661 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
9721 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
9781 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
9841 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
9901 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
9961 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
10021 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
10081 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
10141 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
10201 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
10261 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
10321 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
10381 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
10441 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
10501 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
10561 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
10621 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
10681 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
10741 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
10801 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
10861 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
10921 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
10981 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
11041 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
11101 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
11161 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
11221 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
11281 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
11341 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
11401 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
11461 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
11521 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
11581 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
11641 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
11701 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
11761 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
11821 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
11881 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
11941 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
12001 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
12061 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
12121 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
12181 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
12241 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
12301 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
12361 cct
//
